A study of hybridoma cell growth and antibody production kinetics in continuous culture
β Scribed by K. S. Low; C. Harbour; J. P. Barford
- Publisher
- Springer-Verlag
- Year
- 1987
- Tongue
- English
- Weight
- 465 KB
- Volume
- 1
- Category
- Article
- ISSN
- 0951-208X
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β¦ Synopsis
A detailed study of cell growth and antibody production kinetics in continuous culture found that the specific rate of antibody production reached a maximum saturated profile at a specific growth rate less than the maximum. This observation is novel and of importance in the understanding of the mechanism of antibody production and/or antibody transport.
π SIMILAR VOLUMES
Several experimental data on continuous cultures of hybridoma cells show that monoclonal antibody productivity is a decreasing function of dilution rate. It has been suggested that this unusual behavior may be due to the arrest of a fraction of cycling cells at a critical point of Phase GI. Although
## Abstract The kinetic behavior of a nonproducing hybridoma clone AFPβ27βNP was investigated in continuous culture under glucoseβlimited conditions. A total of more than 21, 000 h of cultures were operated at dilution rates ranging from 0.01 to 0.06 h^β1^. The viable cell concentrations, dead cell