Enhanced production of human monoclonal antibodies by the use of fructose in serum-free hybridoma culture media
β Scribed by Katsumi Mochizuki; Susumu Sato; Masatoshi Kato; Shuichi Hashizume
- Publisher
- Springer
- Year
- 1993
- Tongue
- English
- Weight
- 926 KB
- Volume
- 13
- Category
- Article
- ISSN
- 0920-9069
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Potassium or sodium phosphate was found to stimulate the production of human monoclonal antibody by human-human hybridoma HB4C5. The addition of 15 mM Na-phosphate (pH 7.4) into serum-free culture medium increased the antibody production up to 4-fold, when seeded at cell density of 1 x 10 5 cells/ml
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## Abstract To investigate the effects of factors secreted by different cell lines on human monoclonal antibody (MAb) integrity, 600 mg of a human MAb, which specifically binds to human erythrocytes, were produced in a perfusion process. After purification by protein A affinity chromatography, the