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Immobilization can improve the stability of hybridoma antibody productivity in serum-free media

✍ Scribed by Gyun Min Lee; Bernhard O. Palsson

Publisher: John Wiley and Sons
Year: 1990
Tongue: English
Weight: 666 KB
Volume: 36
Category: Article
ISSN: 0006-3592
DOI: 10.1002/bit.260361010

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✦ Synopsis

Hybridoma cells (S3H5/y2bA2) were cultivated in spinner flasks with 1% serum media and serum-free media. Monoclonal antibody productivity was maintained in 1% serum media. However, cells in serum-free media showed a decrease in antibody productivity, and it completely disappeared in IMDM-based low protein medium. This loss of antibody productivity was not observed when the cells were immobilized in alginate beads. In fact, immobilization enhanced the specific MAb productivity.

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