Stimulation of monoclonal antibody production by human-human hybridoma cells with an elevated concentration of potassium or sodium phosphate in serum-free medium
✍ Scribed by S. Sato; H. Murakami; T. Sugahara; T. Ikegami; K. Yamada; H. Omura; S. Hashizume
- Publisher
- Springer
- Year
- 1989
- Tongue
- English
- Weight
- 325 KB
- Volume
- 2
- Category
- Article
- ISSN
- 0920-9069
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✦ Synopsis
Potassium or sodium phosphate was found to stimulate the production of human monoclonal antibody by human-human hybridoma HB4C5. The addition of 15 mM Na-phosphate (pH 7.4) into serum-free culture medium increased the antibody production up to 4-fold, when seeded at cell density of 1 x 10 5 cells/ml in dishes. At the higher cell density of 5 x 10 5 cells/ml, K-phosphate was more effective than Na-phosphate, at the same concentration. In large-scale continuous culture, the addition of 10 mM Na-phosphate into serum-free culture medium stimulated antibody production by HB4C5 cells 6-fold.