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Cell retention–chemostat studies of hybridoma cells—analysis of hybridoma growth and metabolism in continuous suspension culture in serum-free medium

✍ Scribed by G. W. Hiller; D. S. Clark; H. W. Blanch

Publisher
John Wiley and Sons
Year
1993
Tongue
English
Weight
923 KB
Volume
42
Category
Article
ISSN
0006-3592

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✦ Synopsis

Abstract

The steady‐state metabolic parameters for a hybridoma cell line have been determined in continuous suspension–perfusion culture over a wide range of perfusion rates and cell bleed rates. Significant increases in viable cell concentrations and volumetric productivities were achieved at high perfusion rates and low cell bleed rates. At the low growth rates examined in this study, cellular metabolism shifted to become more oxidative, and as a result, the fraction of consumed substrate converted to inhibitory metabolic by‐products was reduced. Specific antibody productivity was found to be non–growth associated. © 1993 John Wiley & Sons, Inc.

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