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Isolation of an ompC-like outer membrane protein gene from Salmonella typhi

✍ Scribed by Luis Puente, José; Flores, Valia; Fernández, Marcos; Fuchs, Yolanda; Calva, Edmundo

Book ID
122769353
Publisher
Elsevier Science
Year
1987
Tongue
English
Weight
921 KB
Volume
61
Category
Article
ISSN
0378-1119

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Extraction of the outer-membrane porin, OmpC, from Salmonella typhi Ty21a was done by using a modified salt-extraction procedure. It was possible to extract only the major outer-membrane protein (OMP) from the crude membrane using this method. Aberrant lipopolysaccharide (LPS) production in the galE