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Overexpression, refolding, and purification of the major immunodominant outer membrane porin OmpC from Salmonella typhi: characterization of refolded OmpC

✍ Scribed by P.D. Kumar; S. Krishnaswamy

Book ID
116828043
Publisher
Elsevier Science
Year
2005
Tongue
English
Weight
572 KB
Volume
40
Category
Article
ISSN
1046-5928

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Extraction of the outer-membrane porin, OmpC, from Salmonella typhi Ty21a was done by using a modified salt-extraction procedure. It was possible to extract only the major outer-membrane protein (OMP) from the crude membrane using this method. Aberrant lipopolysaccharide (LPS) production in the galE