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Crystallization of the immunodominant outer membrane protein OmpC; the first protein crystals from Salmonella typhi, a human pathogen

✍ Scribed by A Arockiasamy; S Krishnaswamy

Book ID
117111035
Publisher
Elsevier Science
Year
1999
Tongue
English
Weight
170 KB
Volume
453
Category
Article
ISSN
0014-5793

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Extraction of the outer-membrane porin, OmpC, from Salmonella typhi Ty21a was done by using a modified salt-extraction procedure. It was possible to extract only the major outer-membrane protein (OMP) from the crude membrane using this method. Aberrant lipopolysaccharide (LPS) production in the galE