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Functional assay of Salmonella typhi OmpC using reconstituted large unilamellar vesicles: a general method for characterization of outer membrane proteins

✍ Scribed by N. Sundara Baalaji; M.K. Mathew; S. Krishnaswamy

Book ID
116308635
Publisher
Elsevier Science
Year
2006
Tongue
English
Weight
271 KB
Volume
88
Category
Article
ISSN
0300-9084

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✍ A. Arockiasamy; S. Krishnaswamy 📂 Article 📅 2000 🏛 Elsevier Science 🌐 English ⚖ 115 KB

Extraction of the outer-membrane porin, OmpC, from Salmonella typhi Ty21a was done by using a modified salt-extraction procedure. It was possible to extract only the major outer-membrane protein (OMP) from the crude membrane using this method. Aberrant lipopolysaccharide (LPS) production in the galE