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Isolation and purification of ginkgo flavonol glycosides from Ginkgo biloba leaves by high-speed counter-current chromatography

✍ Scribed by Qiang Zhang; Li-Juan Chen; Hao-Yu Ye; Lei Gao; Wenli Hou; Minghai Tang; Guangli Yang; Zhenhua Zhong; Yuan Yuan; Aihua Peng

Publisher
John Wiley and Sons
Year
2007
Tongue
English
Weight
841 KB
Volume
30
Category
Article
ISSN
1615-9306

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✦ Synopsis

Abstract

A high‐speed counter‐current chromatography method was developed for the separation and purification of bioactive flavonol glycosides from a crude ethanol extract of Ginkgo biloba leaves. The separation was performed with a two‐phase solvent system composed of n‐hexane‐butanol‐ethyl acetate‐methanol‐0.5% acetic acid (1:0.5:3.5:1:4, v/v) and three pure compounds were eluted in high purities in a one‐step separation. Their purities were determined by HPLC and identified by MS, ^1^H‐NMR, and ^13^C‐NMR.

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