Preparative isolation and purification of three flavonoid glycosides from Taraxacum mongolicum by high-speed counter-current chromatography

✍ Scribed by Shuyun Shi; Yuping Zhang; Yu Zhao; Kelong Huang

• Publisher: John Wiley and Sons
• Year: 2008
• Tongue: English
• Weight: 845 KB
• Volume: 31
• Category: Article
• ISSN: 1615-9306
• DOI: 10.1002/jssc.200700409

✦ Synopsis

Abstract

A preparative high‐speed counter‐current chromatography (HSCCC) was successively applied to purify three flavonoid glycosides from the aerial part of Taraxacum mongolicum, a traditional Chinese medicine. Subsequent UV, MS, and NMR analyses have led to the characterization of three flavonoid glycosides including two new compounds isoetin‐7‐O‐β‐D‐glucopyranosyl‐2′‐O‐α‐L‐arabinopyranoside and isoetin‐7‐O‐β‐D‐glucopyranosyl‐2′‐O‐α‐D‐glucopyranoside, and a known compound, isoetin‐7‐O‐β‐D‐glucopyranosyl‐2′‐O‐α‐D‐xyloypyranoside, which were first isolated from T. mongolicum. The two‐phase solvent system composed of ethyl acetate/n‐butanol/water (2:1:3, v/v/v) was performed in HSCCC. Consequently, a total of 25.7 mg isoetin‐7‐O‐β‐D‐glucopyranosyl‐2′‐O‐α‐L‐arabinopyranoside, 19.1 mg isoetin‐7‐O‐β‐D‐glucopyranosyl‐2′‐O‐α‐D‐glucopyranoside, and 10.6 mg isoetin‐7‐O‐β‐D‐glucopyranosyl‐2′‐O‐α‐D‐xyloypyranoside were obtained with purity of 98.7, 98.3, and 99.1%, respectively, as determined by HPLC from 500 mg enriched extract after cleaning‐up by polyamide resin.