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Isolation and identification of C-terminal peptides of proteins

✍ Scribed by GU, QU-MING ;SIH, CHARLES J.


Book ID
115099788
Publisher
Wiley (Blackwell Publishing)
Year
2009
Tongue
English
Weight
546 KB
Volume
46
Category
Article
ISSN
0367-8377

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πŸ“œ SIMILAR VOLUMES


Identification of the C-terminal portion
✍ Toshiaki Isobe; Tohru Ichimura; Tsuneo Okuyama πŸ“‚ Article πŸ“… 1986 πŸ› Elsevier Science 🌐 English βš– 494 KB

A method is presented for the simple identification of C-terminal fragment of proteins. The method consists of (i) C-terminal processing of a protein by carboxypeptidase and (ii) comparative peptide mapping of the intact and carboxypeptidase-excised protein after fragmentation by endoproteinase or b

Selective isolation of free and blocked
✍ Harvey Kaplan; George Oda πŸ“‚ Article πŸ“… 1983 πŸ› Elsevier Science 🌐 English βš– 425 KB

A general method for the selective isolation of free and blocked amino-terminal peptides from proteins is described. The rationale behind the methodology is based on the reasoning that if a protein, which has all its free amino groups blocked by citraconylation, is digested with a protease, all pept