Isolation and identification of C-terminal peptides of proteins
β Scribed by GU, QU-MING ;SIH, CHARLES J.
- Book ID
- 115099788
- Publisher
- Wiley (Blackwell Publishing)
- Year
- 2009
- Tongue
- English
- Weight
- 546 KB
- Volume
- 46
- Category
- Article
- ISSN
- 0367-8377
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π SIMILAR VOLUMES
A method is presented for the simple identification of C-terminal fragment of proteins. The method consists of (i) C-terminal processing of a protein by carboxypeptidase and (ii) comparative peptide mapping of the intact and carboxypeptidase-excised protein after fragmentation by endoproteinase or b
A general method for the selective isolation of free and blocked amino-terminal peptides from proteins is described. The rationale behind the methodology is based on the reasoning that if a protein, which has all its free amino groups blocked by citraconylation, is digested with a protease, all pept