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Development of C-terminal Sequencing Analysis of Protein and Peptide

โœ Scribed by Gao Yan-Fei; Wang Hong-Xia


Publisher
Elsevier Science
Year
2007
Tongue
Chinese
Weight
284 KB
Volume
35
Category
Article
ISSN
1872-2040

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We describe the construction of a compact protein sequencer designed specifically for the C-terminal sequence analysis of peptides and proteins. This sequencer has a vertical flow path and is equipped with a continuous flow reactor (CFR). The flow paths for the various reagents and solvents have bee

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The Schlack-Kumpf degradation, also called the isothiocyanate method, is thought to be a promising approach to chemical C-terminal sequencing of peptides and proteins. The derivatizing reagent is most crucial to this method. A new derivatizing reagent, triphenylgermanyl isothiocyanate (TPG-ITC), has