Quantitative protein sequencing using mass spectrometry: Computer-aided assembly of protein sequences from N-terminal peptide sequences

Quantitative protein sequencing using ma

Quantitative protein sequencing using mass spectrometry: thermally induced formation of thiohydantoin amino acid derivatives from N-methyl- and N-phenylthiourea amino acids and peptides in the mass spectrometer

✍ T. Fairwell; S. Ellis; R.E. Lovins 📂 Article 📅 1973 🏛 Elsevier Science 🌐 English ⚖ 365 KB

The thermally induced formation of methyl-and phenylthiohydantoin amino acid derivatives from the corresponding N-methyl-and N-phenylthiourea derivatives of amino acids and peptides has been shown to occur in the mass spectrometer. A comparison of the mass spectra of a number of amino acid thiourea

Identification of two-dimensionally sepa

Identification of two-dimensionally separated human cerebrospinal fluid proteins by N-terminal sequencing, matrix-assisted laser desorption/ionization — mass spectrometry, nanoliquid chromatography-electrospray ionization-time of flight-mass spectrometry, and tandem mass spectrometry

✍ Jos Raymackers; Annick Daniels; Veronique De Brabandere; Carla Missiaen; Martine 📂 Article 📅 2000 🏛 John Wiley and Sons 🌐 English ⚖ 576 KB

Identification of two-dimensionally separated human cerebrospinal fluid proteins by N-terminal sequencing, matrix-assisted laser desorption/ ionization ± mass spectrometry, nanoliquid chromatography-electrospray ionization-time of flight-mass spectrometry, and tandem mass spectrometry Optimal applic

Improved protein identification efficien

Improved protein identification efficiency by mass spectrometry using N-terminal chemical derivatization of peptides from Angiostrongylus costaricensis, a nematode with unknown genome

✍ Ileana R. León; Ana G. C. Neves-Ferreira; Richard H. Valente; Ester M. Mota; Hen 📂 Article 📅 2007 🏛 John Wiley and Sons 🌐 English ⚖ 262 KB

## Abstract Matrix‐assisted laser desorption ionization (MALDI), Peptide Mass Fingerprinting (PMF) and MALDI‐MS/MS ion search (using MASCOT) have become the preferred methods for high‐throughput identification of proteins. Unfortunately, PMF can be ambiguous, mainly when the genome of the organism

Improved protein identification efficien

Improved protein identification efficiency by mass spectrometry using N-terminal chemical derivatization of peptides from Angiostrongylus costaricensis, a nematode with unknown genome

✍ Ileana R. León; Ana G. C. Neves-Ferreira; Richard H. Valente; Ester M. Mota; Hen 📂 Article 📅 2007 🏛 John Wiley and Sons 🌐 English ⚖ 262 KB

## Abstract Matrix‐assisted laser desorption ionization (MALDI), Peptide Mass Fingerprinting (PMF) and MALDI‐MS/MS ion search (using MASCOT) have become the preferred methods for high‐throughput identification of proteins. Unfortunately, PMF can be ambiguous, mainly when the genome of the organism

Structural analysis and identification o

Structural analysis and identification of gel-purified proteins, available in the femtomole range, using a novel computer program for peptide sequence assignment, by matrix-assisted laser desorption ionization — reflectron time-of-fligh — mass spectrometry

✍ Kris Gevaert; Jean-Luc Verschelde; Magda Puype; Jozef Van Damme; Marc Goethals; 📂 Article 📅 1996 🏛 John Wiley and Sons 🌐 English ⚖ 765 KB

Sequencing of N-Linked Oligosaccharides

Sequencing of N-Linked Oligosaccharides Directly from Protein Gels: In-Gel Deglycosylation Followed by Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry and Normal-Phase High-Performance Liquid Chromatography

✍ Bernhard Küster; Susan F. Wheeler; Ann P. Hunter; Raymond A. Dwek; David J. Harv 📂 Article 📅 1997 🏛 Elsevier Science 🌐 English ⚖ 580 KB