The application of high performance liquid chromatography (HPLC) to separate components in solvent suspension of Escherichia coli followed by off-line matrix-assisted laser desorption/ionization (MALDI) analysis of collected fractions results in the detection of over 300 peaks in the 2000-19,000 Da
✦ LIBER ✦
Sequencing of N-Linked Oligosaccharides Directly from Protein Gels: In-Gel Deglycosylation Followed by Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry and Normal-Phase High-Performance Liquid Chromatography
✍ Scribed by Bernhard Küster; Susan F. Wheeler; Ann P. Hunter; Raymond A. Dwek; David J. Harvey
- Publisher
- Elsevier Science
- Year
- 1997
- Tongue
- English
- Weight
- 580 KB
- Volume
- 250
- Category
- Article
- ISSN
- 0003-2697
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Non-porous reversed phase (NPRP) high-performance liquid chromatography (HPLC) has been used as a rapid method to separate proteins from whole cell lysates of human erythroleukemia (HEL) cells. Using phosphate-buffered saline (PBS) as a lysis buffer to extract proteins from HEL cells, more than 100