✦ LIBER ✦

Detection and identification of low-mass peptides and proteins from solvent suspensions of Escherichia coli by high performance liquid chromatography fractionation and matrix-assisted laser desorption/ionization mass spectrometry

✍ Scribed by Yuqin Dai; Liang Li; Dennis C. Roser; S. Randolph Long

Publisher: John Wiley and Sons
Year: 1999
Tongue: English
Weight: 84 KB
Volume: 13
Category: Article
ISSN: 0951-4198
DOI: 10.1002/(sici)1097-0231(19990115)13:1<73::aid-rcm454>3.0.co;2-n

No coin nor oath required. For personal study only.

✦ Synopsis

The application of high performance liquid chromatography (HPLC) to separate components in solvent suspension of Escherichia coli followed by off-line matrix-assisted laser desorption/ionization (MALDI) analysis of collected fractions results in the detection of over 300 peaks in the 2000-19,000 Da mass range, an order of magnitude increase in the number of components observed when compared with direct MALDI analysis of the entire solvent suspension. Mass measurements of these separated components using a timelag focusing MALDI instrument are reported. MALDI analysis of the proteolytic digests of several collected fractions facilitates the identification of three components as specific proteins expected to be present in E. coli. The methodologies reported here should be very useful in searching for unique biomarkers for bacterial discrimination.

📜 SIMILAR VOLUMES

Protein profiles and identification of h

Protein profiles and identification of high performance liquid chromatography isolated proteins of cancer cell lines using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry

✍ Bathsheba E. Chong; David M. Lubman; Allen Rosenspire; Fred Miller 📂 Article 📅 1998 🏛 John Wiley and Sons 🌐 English ⚖ 119 KB 👁 2 views

Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOFMS) has been used to rapidly profile the protein content of human cell lysates from MCF-10 cell and variant lines. The method was used to study the protein profiles of these cells as they progressed from normal br

High-resolution Mass Spectrometry and Ac

High-resolution Mass Spectrometry and Accurate Mass Measurements with Emphasis on the Characterization of Peptides and Proteins by Matrix-assisted Laser Desorption/Ionization Time-of-flight Mass Spectrometry

✍ Russell, David H.; Edmondson, Ricky D. 📂 Article 📅 1997 🏛 John Wiley and Sons 🌐 English ⚖ 434 KB 👁 1 views

Fundamental concepts of high-resolution mass spectrometry as it relates to the issue of accurate mass measurements are presented. Important issues speciÐc to magnetic sector instruments, Fourier transform ion cyclotron resonance and time-of-Ñight (TOF) methods are discussed. Recent high-resolution T

Multidimensional peptide fingerprinting

Multidimensional peptide fingerprinting by high performance liquid chromatography, capillary zone electrophoresis and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry for the identification of tarantula venom samples

✍ Pierre Escoubas; Brian J. Whiteley; Charles P. Kristensen; Marie-Louise Célérier 📂 Article 📅 1998 🏛 John Wiley and Sons 🌐 English ⚖ 236 KB 👁 2 views

The increased interest in spider venom toxins has made precise identification of venom samples necessary for reproducibility of biochemical and pharmacological studies. In the family Theraphosidae (Tarantulas), identification of specimens may be difficult and a new approach involving multidimensiona

High-performance liquid chromatography m

High-performance liquid chromatography matrix-assisted laser desorption/ionization time-of-flight mass spectrometry peptide fingerprinting of tarantula venoms in the genus Brachypelma: chemotaxonomic and biochemical applications

✍ Pierre Escoubas; Marie-Louise Célérier; Terumi Nakajima 📂 Article 📅 1997 🏛 John Wiley and Sons 🌐 English ⚖ 225 KB 👁 2 views

Precise identification of arthropod species is fundamental in venom research, particularly in groups where taxonomy problems remain unsolved. High-performance liquid chromatography and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOFMS) analysis of crude venoms

Analysis of intact proteins from cerebro

Analysis of intact proteins from cerebrospinal fluid by matrix-assisted laser desorption/ionization mass spectrometry after two-dimensional liquid-phase electrophoresis

✍ Maja Puchades; Ann Westman; Kaj Blennow; Pia Davidsson 📂 Article 📅 1999 🏛 John Wiley and Sons 🌐 English ⚖ 76 KB 👁 1 views

A novel combination of methods, two-dimensional liquid-phase electrophoresis (2D-LPE) and matrixassisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOFMS), have been used for the analysis of intact brain-specific proteins in cerebrospinal fluid (CSF). 2D-LPE is especially us

Rapid identification and screening of pr

Rapid identification and screening of proteins from whole cell lysates of human erythroleukemia cells in the liquid phase, using non-porous reversed phase high-performance liquid chromatography separations of proteins followed by multi-assisted laser desorption/ionization mass spectrometry analysis and sequence database searching

✍ Yajuan Chen; Dan Wall; David M. Lubman 📂 Article 📅 1998 🏛 John Wiley and Sons 🌐 English ⚖ 97 KB 👁 1 views

Non-porous reversed phase (NPRP) high-performance liquid chromatography (HPLC) has been used as a rapid method to separate proteins from whole cell lysates of human erythroleukemia (HEL) cells. Using phosphate-buffered saline (PBS) as a lysis buffer to extract proteins from HEL cells, more than 100