Identification of C-terminal residues in peptides and proteins through formation of thiohydantoins
โ Scribed by Robert A. Turner; Gertrude Schmerzler
- Book ID
- 115785987
- Publisher
- Elsevier Science
- Year
- 1954
- Weight
- 513 KB
- Volume
- 13
- Category
- Article
- ISSN
- 0006-3002
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๐ SIMILAR VOLUMES
Proteins or peptides having an \(\mathrm{N}\)-terminal-blocked amino acid were successively digested by pronase \(E\), proteinase \(K\), and carboxypeptidase \(Y\). The \(\mathbf{N}\)-blocked amino acids released from proteins or peptides were derivatized with 9 -anthryldiazomethane (ADAM) to the co
A method is presented for the simple identification of C-terminal fragment of proteins. The method consists of (i) C-terminal processing of a protein by carboxypeptidase and (ii) comparative peptide mapping of the intact and carboxypeptidase-excised protein after fragmentation by endoproteinase or b