A PREPARATIVE METHOD FOR THE ISOLATION OF CARBOXYL TERMINAL TRYPTIC PEPTIDES FROM PROTEINS
β Scribed by Hargrave, Paul A. ;Wold, Finn
- Book ID
- 115097044
- Publisher
- Wiley (Blackwell Publishing)
- Year
- 2009
- Tongue
- English
- Weight
- 357 KB
- Volume
- 5
- Category
- Article
- ISSN
- 0367-8377
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π SIMILAR VOLUMES
A method for selective isolation of the amino \(\mathrm{N}\)-terminal peptide from an \(\alpha\)-amino \(\left(N^{\alpha}\right.\) )-blocked protein is presented. The method consists of four steps. First, \(\epsilon-\) amino groups of lysine residues in the protein are succinylated. Second, the deri
A novel method useful for selective isolation of the C-terminal peptide from a tryptic digestion mixture of a protein has been developed by taking advantage of a unique property of anhydrotrypsin, which has a strong specific affinity for the peptides containing arginine or lysine at their C-termini.