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PREPARATION OF CARBOXYL-TERMINAL TRYPTIC PEPTIDES FROM PROTEINS BY CLEAVAGE AT ARGININE

โœ Scribed by Fong, Shao-Ling ;Hargrave, Paul A.


Book ID
115097304
Publisher
Wiley (Blackwell Publishing)
Year
2009
Tongue
English
Weight
479 KB
Volume
10
Category
Article
ISSN
0367-8377

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A novel method useful for selective isolation of the C-terminal peptide from a tryptic digestion mixture of a protein has been developed by taking advantage of a unique property of anhydrotrypsin, which has a strong specific affinity for the peptides containing arginine or lysine at their C-termini.