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Isolation and culture of sinusoidal cells from rat and human liver needle biopsies

Book ID
122613855
Publisher
Elsevier Science
Year
1985
Tongue
English
Weight
66 KB
Volume
1
Category
Article
ISSN
0168-8278

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Isolation, Culture and Characterization
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A technique is described for isolation and culture of adult human hepatocytes from surgical liver biopsies. The mean cell yield was 1.75 X 10(7) cells per gm liver and viability averaged 80%. Hepatocytes were maintained in primary culture for about 10 days. Cell morphology and histochemical characte