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Isolation, Culture and Characterization of Adult Human Hepatocytes from Surgical Liver Biopsies

✍ Scribed by Francois Ballet; Marie-Elisabeth Bouma; Shu-Ren Wang; Norma Amit; Jacqueline Marais; Recaredo Infante

Publisher: John Wiley and Sons
Year: 1984
Tongue: English
Weight: 788 KB
Volume: 4
Category: Article
ISSN: 0270-9139
DOI: 10.1002/hep.1840040509

No coin nor oath required. For personal study only.

✦ Synopsis

A technique is described for isolation and culture of adult human hepatocytes from surgical liver biopsies. The mean cell yield was 1.75 X 10(7) cells per gm liver and viability averaged 80%. Hepatocytes were maintained in primary culture for about 10 days. Cell morphology and histochemical characteristics were similar to hepatocytes in vivo. Bile canaliculi were observed by electron microscopy. Intracellular albumin was demonstrated up to the 7th day of culture; albumin secretion rate was maximal (0.6 +/- 0.33 micrograms per hr per 10(6) cells) 5 days after plating. These studies demonstrate that adult human hepatocytes can be isolated from surgical biopsies with high yield, and differentiated function can be maintained for several days.

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