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Characterization of adult human astrocytes derived from explant culture

✍ Scribed by M. L. Estes; R. M. Ransohoff; J. T. McMahon; B. S. Jacobs; B. P. Barna

Publisher: John Wiley and Sons
Year: 1990
Tongue: English
Weight: 982 KB
Volume: 27
Category: Article
ISSN: 0360-4012
DOI: 10.1002/jnr.490270430

No coin nor oath required. For personal study only.

✦ Synopsis

Center for Multiple Sclerosis Treatment and Research (R.M.R.), Cleveland Clinic Foundation Four different human astrocytic cell lines established from either epilepsy surgical specimens or cerebral white matter obtained during thalamotomy for tremor in a patient with multiple sclerosis were characterized using morphologic analysis, ultrastructural attributes, growth characteristics, and immunocytochemical analysis. Immunocytochemical characterization of cultures indicated a mean of 84% of cells contained cytoplasmic glial fibrillary acidic protein (GFAP): to confirm that GFAP( + ) cells also proliferated, bromo-deoxyuridine (BrdU) uptake was measured in cell line. Our method of simplified explant culture allows establishment of astrocytic cell lines from a variety of pathologic substrates using limited amounts of human material.

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