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Isolation and characterization of human osteoblasts from needle biopsies without in vitro culture

โœ Scribed by Fujita, K.; Roforth, M. M.; Atkinson, E. J.; Peterson, J. M.; Drake, M. T.; McCready, L. K.; Farr, J. N.; Monroe, D. G.; Khosla, S.

Book ID
121553064
Publisher
Springer-Verlag
Year
2013
Tongue
English
Weight
379 KB
Volume
25
Category
Article
ISSN
0937-941X

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A technique is described for isolation and culture of adult human hepatocytes from surgical liver biopsies. The mean cell yield was 1.75 X 10(7) cells per gm liver and viability averaged 80%. Hepatocytes were maintained in primary culture for about 10 days. Cell morphology and histochemical characte