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Large-Scale Isolation of Sinusoidal Endothelial Cells from Pig and Human Liver

✍ Scribed by Jörg C. Gerlach; Katrin Zeilinger; Gabriele Spatkowski; Florian Hentschel; Norbert Schnoy; Stefan Kolbeck; Rudolf K. Schindler; Peter Neuhaus

Book ID
115636461
Publisher
Elsevier Science
Year
2001
Tongue
English
Weight
573 KB
Volume
100
Category
Article
ISSN
0022-4804

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A laboratory process for the isolation of type B acid phosphatase from human erythrocytes has been scaled up 80 times. The enzyme was purified 1300-fold by adsorption on calcium phosphate gel, ammonium sulfate precipitation, and gel filtration. The yield was 277,. Results for the type A enzyme are a