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Large-Scale isolation of acid phosphatase from human erythrocytes

✍ Scribed by R. A. Fisher; H. Harris; M. Houldsworth; M. D. Lilly; P. Dunnill

Publisher
John Wiley and Sons
Year
1968
Tongue
English
Weight
691 KB
Volume
10
Category
Article
ISSN
0006-3592

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✦ Synopsis

A laboratory process for the isolation of type B acid phosphatase from human erythrocytes has been scaled up 80 times. The enzyme was purified 1300-fold by adsorption on calcium phosphate gel, ammonium sulfate precipitation, and gel filtration. The yield was 277,. Results for the type A enzyme are also presented.

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