A procedure is described that facilitates the isolation of large quantities of nuclei (germinal vesicles or GVs) from late stage Xenopus laevis oocytes. The method was developed by modifying techniques described in two published reports (F. Scalenghe et al. (1978) Chromosoma 66, 299-308; I. Ruberti
Large scale isolation of nuclei from oocytes of Xenopus laevis
โ Scribed by Ida Ruberti; Elena Beccari; Elisabetta Bianchi; Francesca Carnevali
- Publisher
- Elsevier Science
- Year
- 1989
- Tongue
- English
- Weight
- 459 KB
- Volume
- 180
- Category
- Article
- ISSN
- 0003-2697
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## Abstract The buoyant density of melanosomes isolated from oocytes of __Xenopus laevis__ is about 1.88 g/cm^3^ while most of the melanosomes synthesized in the larvae have a density of about 1.92 g/cm^3^. The melanosomes isolated from phenylthioureaโtreated larvae or from their eyes have a densit