✦ LIBER ✦

Isolation and characterization of spontaneous srl-recA deletion mutants in Escherichia coli K-12

✍ Scribed by Turner, Peter C. ;Oliver, Philip

Publisher: Springer
Year: 1984
Tongue: English
Weight: 465 KB
Volume: 196
Category: Article
ISSN: 0026-8925
DOI: 10.1007/bf00328071

No coin nor oath required. For personal study only.

✦ Synopsis

A method was developed for the isolation of spontaneous mutants of Escherichia coli K-12 with deletions extending from the srl operon to the adjacent recA gene. The srl-recA deletion mutants were extremely sensitive to dNA-damaging agents; unable to support growth of the feckless red gam mutant lambda bio11; and recombination-deficient in transduction and in conjugation. They therefore resembled recA point mutants such as recA13. The existence of these recA deletion mutants shows that the recA gene is not essential for viability.

📜 SIMILAR VOLUMES

Isolation and characterization of a new

Isolation and characterization of a new type of Escherichia coli K12 phoB mutants

✍ Heyde, Martine ;Portalier, Raymond 📂 Article 📅 1983 🏛 Springer 🌐 English ⚖ 844 KB

A new type of Escherichia coli K12 phoB mutant was isolated as 5-fluorouracil-plus-adenosine-resistant derivatives of a upp phoS,T strain. Such phoB mutants (called type III) differ from previously described phoB mutants (types I and II) in the synthesis pattern of phosphate-regulated periplasmic pr

Isolation and characterization of a temp

Isolation and characterization of a temperature-sensitive amber suppressor mutant of Escherichia coli K12

✍ Nagata, Toshio ;Horiuchi, Takashi 📂 Article 📅 1973 🏛 Springer 🌐 English ⚖ 719 KB

Isolation and characterization of mutant

Isolation and characterization of mutants with a feedback resistant N-acetylglutamate synthase in Escherichia coli K 12

✍ Eckhardt, Thomas ;Leisinger, Thomas 📂 Article 📅 1975 🏛 Springer 🌐 English ⚖ 493 KB

Mutants with a feedback resistant N-acetylglutamate synthase have been isolated from a proA/B, argD, argR strain by screening for proline excretion on minimal medium with arginine. The feedback resistant character of three mutants was transduced into an argA (N-acetylglutamate synthase negative) str

Isolation and mapping of Escherichia col

Isolation and mapping of Escherichia coli K12 mutants defective in Tn9 transposition

✍ Hyina, T. S. ;Nechaeva, E. V. ;Romanova, Yu. M. ;Smirnov, G. B. 📂 Article 📅 1981 🏛 Springer 🌐 English ⚖ 521 KB

Five mutants (called tnm) of Escherichia coli with impaired ability for transposition of Tn9 were isolated after treatment with ethyl methanesulfonate (EMS) or N-methyl-N'-nitro-N-nitrosoguanidine (NG). The map locations of the tnm mutations were determined by a combination of Hfr matings, F' episom

Genetic characterization of streptomycin

Genetic characterization of streptomycin-resistant and-dependent mutants of Escherichia coli K12

✍ Sánchez-Anzaldo, F. J. ;Bastarrachea, Fernando 📂 Article 📅 1974 🏛 Springer 🌐 English ⚖ 1003 KB

The recQ gene of Escherichia coli K12: m

The recQ gene of Escherichia coli K12: molecular cloning and isolation of insertion mutants

✍ Nakayama, Koji ;Irino, Nobuto ;Nakayama, Hiroaki 📂 Article 📅 1985 🏛 Springer 🌐 English ⚖ 569 KB

The recQ gene of Escherichia coli K12 was subcloned from plasmid pKO1 (Oeda et al. 1981) by monitoring the capacity of the resulting recombinant plasmids partially to reverse the increased ultraviolet (UV) sensitivity of a reeF143 recQ1 double mutant. We were able to trace this complementation activ