𝔖 Bobbio Scriptorium
✦   LIBER   ✦

Isolation and characterization of mutants with a feedback resistant N-acetylglutamate synthase in Escherichia coli K 12

✍ Scribed by Eckhardt, Thomas ;Leisinger, Thomas

Publisher
Springer
Year
1975
Tongue
English
Weight
493 KB
Volume
138
Category
Article
ISSN
0026-8925

No coin nor oath required. For personal study only.

✦ Synopsis

Mutants with a feedback resistant N-acetylglutamate synthase have been isolated from a proA/B, argD, argR strain by screening for proline excretion on minimal medium with arginine. The feedback resistant character of three mutants was transduced into an argA (N-acetylglutamate synthase negative) strain. It was cotransducible with argA at a frequency of greater than 99%. N-acetylglutamate synthase extracted from the three mutants was approximately one hundred times less sensitive to L-arginine than the enzyme from the feedback sensitive parent strain.

πŸ“œ SIMILAR VOLUMES

Isolation and characterization of a new
Isolation and characterization of a new type of Escherichia coli K12 phoB mutants
✍ Heyde, Martine ;Portalier, Raymond πŸ“‚ Article πŸ“… 1983 πŸ› Springer 🌐 English βš– 844 KB

A new type of Escherichia coli K12 phoB mutant was isolated as 5-fluorouracil-plus-adenosine-resistant derivatives of a upp phoS,T strain. Such phoB mutants (called type III) differ from previously described phoB mutants (types I and II) in the synthesis pattern of phosphate-regulated periplasmic pr

Isolation and characterization of sponta
Isolation and characterization of spontaneous srl-recA deletion mutants in Escherichia coli K-12
✍ Turner, Peter C. ;Oliver, Philip πŸ“‚ Article πŸ“… 1984 πŸ› Springer 🌐 English βš– 465 KB

A method was developed for the isolation of spontaneous mutants of Escherichia coli K-12 with deletions extending from the srl operon to the adjacent recA gene. The srl-recA deletion mutants were extremely sensitive to dNA-damaging agents; unable to support growth of the feckless red gam mutant lamb

Isolation and genetic characterization o
Isolation and genetic characterization of a thymineless death-resistant mutant of Escherichia coli K12: Identification of a new mutation (recQ1) that blocks the RecF recombination pathway
✍ Nakayama, Hiroaki ;Nakayama, Koji ;Nakayama, Ritsuko ;Irino, Nobuto ;Nakayama, Y πŸ“‚ Article πŸ“… 1984 πŸ› Springer 🌐 English βš– 640 KB

An Escherichia coli K12 mutant resistant to thymineless death (TLD) was isolated, and its genetic analysis led us to identify a new mutation (recQ1) located between corA and metE on the standard linkage map. The mutation was found to result in increased sensitivity to ultraviolet light and deficienc