Isolation and characterization of a new type of Escherichia coli K12 phoB mutants

✍ Scribed by Heyde, Martine ;Portalier, Raymond

• Publisher: Springer
• Year: 1983
• Tongue: English
• Weight: 844 KB
• Volume: 190
• Category: Article
• ISSN: 0026-8925
• DOI: 10.1007/bf00330333

✦ Synopsis

A new type of Escherichia coli K12 phoB mutant was isolated as 5-fluorouracil-plus-adenosine-resistant derivatives of a upp phoS,T strain. Such phoB mutants (called type III) differ from previously described phoB mutants (types I and II) in the synthesis pattern of phosphate-regulated periplasmic proteins P4a and 30.5 K, sensitivity to toxic compounds, and several other phenotypic characters. The analysis of isogenic strains carrying phoB mutations (type I, II or III) showed that; the phoB gene product exerted (i) a positive control over the synthesis of periplasmic proteins 30.5 K, 11.5 K, and 9 K, inner membrane proteins 32 K and 17.5 K, and outer membrane protein Tsx, (ii) and a direct or indirect negative control over the synthesis of a 20 K phosphate-inducible periplasmic protein.