✦ LIBER ✦

Isolation and characterization of 5T4, a tumour-associated antigen

✍ Scribed by Nicholas Hole; Peter L. Stern

Publisher: John Wiley and Sons
Year: 1990
Tongue: French
Weight: 806 KB
Volume: 45
Category: Article
ISSN: 0020-7136
DOI: 10.1002/ijc.2910450132

No coin nor oath required. For personal study only.

✦ Synopsis

The monoclonal antibody (MAb) 514 defines a human trophoblast antigen marker with a restricted pattern of expression in normal adult tissues but this antigen is expressed on a variety of carcinomas. The purification of 5T4 antigenic molecules is described from term syncytiotrophoblast by a combination of lectin-and immunoaffinity chromatography and gel filtration giving up to 10,000-fold purification with 70% yield. The antigen is carried by non-associated glycoprotein molecules with an apparent molecular weight of 72 kDa on SDS-PAGE and a neutral pl. Removal of N-linked sugars by N-glycanase reveals a core protein of 42 kDa. Treatment with enzymes that cleave 0-linked sugars does not substantially alter the molecular size. The native 5T4 molecules are very resistant to proteolysis until the N-linked sugars are removed or the glycoprotein is denatured and reduced. Glycopeptides generated by these approaches will be suitable for amino acid sequencing.

3T0 whom reprint requests should be addressed.

📜 SIMILAR VOLUMES

Characterization, isolation and amino te

Characterization, isolation and amino terminal sequencing of a rat colon carcinoma-associated antigen

✍ Corinne Chadeneau; Marc G. Denis; Hervé M. Blottière; Marc Grégoire; Jean-Yves D 📂 Article 📅 1991 🏛 John Wiley and Sons 🌐 French ⚖ 798 KB

Monoclonal antibodies have been raised against a cell line derived from a dimethylhydrazine-induced rat colon carcinoma. One of these antibodies (MAb E4) has previously been shown to react slightly with normal small intestine and colon, and not with other normal tissues as determined by immunohistoc

Isolation and characterization of a sero

Isolation and characterization of a serologically defined tumour membrane antigen from a chemically-induced rat sarcoma, HSN

✍ Olivier Léger; Pamela L. Dunn; B. Coles; C. J. Dean 📂 Article 📅 1991 🏛 John Wiley and Sons 🌐 French ⚖ 819 KB

Polypeptides containing the tumour antigenic determinant present on the external domain of a membrane antigen of the 3-4 benzpyrene-induced rat fibrosarcoma HSN have been isolated and purified. Following cleavage from intact cells with trypsin, the peptides were purified by irnmunoaffinity chromatog

Isolation of membrane-associated tumour-

Isolation of membrane-associated tumour-specific antigen from an aminoazo-dye-induced rat hepatoma

✍ R. W. Baldwin; M. Moore 📂 Article 📅 1969 🏛 John Wiley and Sons 🌐 French ⚖ 532 KB

10' cells per ml antiserum. Membrane equivalent loB cells per ml antiserum.

Cd8 T-cell recognition of human 5T4 onco

Cd8 T-cell recognition of human 5T4 oncofetal antigen

✍ Lucy JC Smyth; Eyad Elkord; Taher E.I. Taher; Hui-Rong Jiang; Deborah J. Burt; A 📂 Article 📅 2006 🏛 John Wiley and Sons 🌐 French ⚖ 316 KB

## Abstract The 5T4 oncofetal antigen is expressed by a wide variety of human carcinomas, including colorectal, ovarian and gastric carcinomas. The restricted expression of 5T4 on tumor tissues as well as its implication in tumor progression and bad prognosis makes 5T4 a promising new candidate for

Isolation of a soluble tumor-associated

Isolation of a soluble tumor-associated antigen from human melanoma

✍ Jack A. Roth; E. Carmack Holmes; Ralph A. Reisfeld; Harry K. Slocum; Donald L. M 📂 Article 📅 1976 🏛 John Wiley and Sons 🌐 English ⚖ 573 KB

a n melanoma-associated antigen was solubilized from fresh surgical specimens by 3 M KCl extraction. T h e antigenicity of this extract was demonstrated by delayed cutaneous hypersensitivity responses and inhibition of complement fixation. Twenty-one of 33 melanoma patients had delayed cutaneous hyp

Primary melanoma tumour regression assoc

Primary melanoma tumour regression associated with an immune response to the tumour-associated antigen melan-A/MART-1

✍ Farid H. Saleh; Kerry A. Crotty; Peter Hersey; Scott W. Menzies 📂 Article 📅 2001 🏛 John Wiley and Sons 🌐 French ⚖ 418 KB

A prediction of the theory of immunologic surveillance is that tumour antigens can be recognised by cell-mediated immunity during early development of the primary tumour by formation of tumour antigen-specific cytotoxic lymphocytes (CTLs) and that such recognition leads to destruction of those tumou