Isolation of membrane-associated tumour-specific antigen from an aminoazo-dye-induced rat hepatoma
✍ Scribed by R. W. Baldwin; M. Moore
- Publisher
- John Wiley and Sons
- Year
- 1969
- Tongue
- French
- Weight
- 532 KB
- Volume
- 4
- Category
- Article
- ISSN
- 0020-7136
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✦ Synopsis
10' cells per ml antiserum.
Membrane equivalent loB cells per ml antiserum.
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## Abstract Deletion of liver‐specific cell‐surface‐associated antigens from aminoazodyeinduced rat hepatomas was demonstrated by immunofluorescence techniques. Each of the hepatomas studied was found to lack a characteristic individual normal liver antigen. This parallels the concomitant expressio
## Abstract Cellular and humoral immune reactions against tumour‐specific antigens on aminoazo‐dye‐induced rat hepatomas were demonstrated __in vitro__ by the colony inhibition technique. Lymph‐node cells from syngeneic rats immunized against individual hepatomas inhibited colony formation by cells
Polypeptides containing the tumour antigenic determinant present on the external domain of a membrane antigen of the 3-4 benzpyrene-induced rat fibrosarcoma HSN have been isolated and purified. Following cleavage from intact cells with trypsin, the peptides were purified by irnmunoaffinity chromatog