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Isolation and characterization of a serologically defined tumour membrane antigen from a chemically-induced rat sarcoma, HSN

✍ Scribed by Olivier Léger; Pamela L. Dunn; B. Coles; C. J. Dean

Publisher
John Wiley and Sons
Year
1991
Tongue
French
Weight
819 KB
Volume
47
Category
Article
ISSN
0020-7136

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✦ Synopsis

Polypeptides containing the tumour antigenic determinant present on the external domain of a membrane antigen of the 3-4 benzpyrene-induced rat fibrosarcoma HSN have been isolated and purified. Following cleavage from intact cells with trypsin, the peptides were purified by irnmunoaffinity chromatography and SDS-PAGE. Three polypeptides of molecular weight 120,45 and 42 kDa were obtained that bound the specific rat monoclonal antibody (MAb) 11/160 in Western blots. Chemical analyses of the 45-kDa fragment yielded the following sequence:

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