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Isolation of a soluble tumor-associated antigen from human melanoma

✍ Scribed by Jack A. Roth; E. Carmack Holmes; Ralph A. Reisfeld; Harry K. Slocum; Donald L. Morton

Publisher
John Wiley and Sons
Year
1976
Tongue
English
Weight
573 KB
Volume
37
Category
Article
ISSN
0008-543X

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✦ Synopsis

a n melanoma-associated antigen was solubilized from fresh surgical specimens by 3 M KCl extraction. T h e antigenicity of this extract was demonstrated by delayed cutaneous hypersensitivity responses and inhibition of complement fixation. Twenty-one of 33 melanoma patients had delayed cutaneous hypersensitivity responses to melanoma antigen, while only four of 28 reacted to autologous muscle. Although KCI extracts did not fix complement directly, they reacted with antibody, thus inhibiting complement fixation by the autologous melanoma antigen extracted from tissue culture supernatants. T h e soluble antigenic moiety was then purified by fractionation on a G-150 Sephedex column and polyacrylamide gels, a n d the antigenic activity was monitored by delayed cutaneous hypersensitivity responses in melanoma patients. A 20-fold purification wan achieved. Solubilization of tumor antigens with 3 M KCl provides tumor-associated antigen of high activity which is amenable to further biochemical purification.

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