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Detection of tumor-associated antigen in human melanoma cell line supernatants

✍ Scribed by Elizabeth A. Grimm; Hulbert K. B. Silver; Jack A. Roth; Darwin O. Chee; Rishab K. Gupta; Donald L. Morton

Publisher: John Wiley and Sons
Year: 1976
Tongue: French
Weight: 477 KB
Volume: 17
Category: Article
ISSN: 0020-7136
DOI: 10.1002/ijc.2910170503

No coin nor oath required. For personal study only.

✦ Synopsis

Abstract

Spent tissue culture medium (CDM‐S) removed from a single cell line of human malignant melanoma grown in serum‐free CDM, contained tumor‐associated antigenic activity. Antibodies to CDM‐S measured by complement fixation were detected in 44% (31/70) melanoma, 55% (15/27) sarcoma, 63% (24/38) carcinoma and 15% (11/72) normal sera. Delayed cutaneous hypersensitivity reactions (DCHR) were demonstrated in 4/5 melanoma patients at a 500 μg dose, 3/5 at a 100 μg dose and in 1/7 carcinoma patients at the 500 μg dose. One ml of CDM‐S was shown to contain antigen equivalent to that obtained from the membranes of 2.9 × 10^7^ tissue‐cultured melanoma cells. After purification, 84% (16/19) sera from melanoma patients, 66% (12/18) from sarcoma and carcinoma patients and 8% (2/26) from normal controls were positive to the antigen by complement fixation.

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