For a quick analytical identification of histones a two-dimensional electrophoretic system has been developed. First the proteins are separated on cellulose acetate strips in alkaline buffer. Then they are reelectrophoresed in a second dimension on polyacrylamide gels either with sodium dodecyl sulf
β¦ LIBER β¦
Ion-exchange electrophoresis of histone on polyacrylamide gel containing acrylic acid
β Scribed by J.R. Joice; H.G. Klemperer
- Publisher
- Elsevier Science
- Year
- 1971
- Tongue
- English
- Weight
- 845 KB
- Volume
- 41
- Category
- Article
- ISSN
- 0003-2697
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We have developed a method for the efficient transfer of histones from acetic acid-urea-Triton X-100 (AUT)-polyacrylamide minislab gels to nitrocellulose. The AUT gel was equilibrated with 50 mM acetic acid and 0.5% sodium dodecyl sulfate and then with 62.5 mM Tris-HCl, pH 6.8, and 2.3% sodium dodec