Two-dimensional electrophoresis of yeast histones on cellogel strips and polyacrylamide gels

✍ Scribed by Martin Veth; Johann Sonnenbichler

• Publisher: Elsevier Science
• Year: 1986
• Tongue: English
• Weight: 522 KB
• Volume: 153
• Category: Article
• ISSN: 0003-2697
• DOI: 10.1016/0003-2697(86)90060-6

✦ Synopsis

For a quick analytical identification of histones a two-dimensional electrophoretic system has been developed. First the proteins are separated on cellulose acetate strips in alkaline buffer. Then they are reelectrophoresed in a second dimension on polyacrylamide gels either with sodium dodecyl sulfate or in the presence of urea at pH 2.7. The technique is demonstrated with yeast and calf thymus histones. 0 1986 academic PM, h.