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Ion chromatography for monitoring biotechnological processes: Part I. System development for analysis of industrial cultivation media of microorganisms

✍ Scribed by Ute Scheller; Detlef Siedenberg; Karl Schügerl

Publisher
Elsevier Science
Year
1994
Tongue
English
Weight
599 KB
Volume
285
Category
Article
ISSN
0003-2670

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✦ Synopsis

An ion-exchange chromatographic method was developed for measuring chloride, phosphate, nitrate, and sulfate anions in model media, and chloride, phosphate and sulfate anions in industrial cultivation medii of microorganisms. By optimisation, an analysis time of 14 min was obtained. An ion-exclusion chromatographic method was developed for the measurement of glucose, lactic, acetic and citric acid concentrations in model and industrial cultivation media of microorganisms. By optimisation, an analysis time of 10 min was reached. The prerequisite of these analyses is proper sample preparation, the removal of proteins from the sample in particular, which is discussed in detail in this publication.

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