Ion chromatography for monitoring biotechnological processes: Part II. Applications for industrial cultivation media

✍ Scribed by Ute Scheller; Detlef Siedenberg; Uwe Hübner; Michael Siebold; Gerlinde Kretzmer; Karl Schügerl

• Publisher: Elsevier Science
• Year: 1994
• Tongue: English
• Weight: 462 KB
• Volume: 286
• Category: Article
• ISSN: 0003-2670
• DOI: 10.1016/0003-2670(94)80158-4

✦ Synopsis

Ion-exchange chromatography was applied for off-line and on-line monitoring of chloride, phosphate and sulfate anions during the production of alkaline protease by Bacillus licheniformis and during the cultivation of Cephalosporium acremonium . Ion-exclusion chromatography was used for off-tine and on-line monitoring of acetic, citric and lactic acid anions as well as glucose during the production of lactic acid by Lactobacillus salivarius subsp . salivarius . The problems of sampling and sample pretreatment and optimization of the chromatographic analysis and enrichment of the analytes after dialysis are discussed .