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Development of enzyme cartridge flow-injection analysis for industrial process monitoring. Part II. Application for monitoring of microorganism cultivations

✍ Scribed by H. Jürgens; L. Brandes; R. Joppien; M. Siebold; J. Schubert; X. Wu; G. Kretzmer; K. Schügerl

Publisher
Elsevier Science
Year
1995
Tongue
English
Weight
615 KB
Volume
302
Category
Article
ISSN
0003-2670

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✦ Synopsis

Five cartridges with immobilized glucose oxydase, alcohol oxydase, pyranose oxydase, L-amino acid oxydase and t_-lactate-2-monooxigenase, respectively, were integrated into a flow injection analysis (FIA) system and applied to the monitoring of medium components: glucose and ethanol during the cultivation of Saccharomyces cereuisiae, L-amino acids during the production of the fusion protein EcoRI::SPA by recombinant Escherichiu coli, and lactate during the production of lactic acid by Luctobucillus saliuurius var. saliuurius. The developed enzyme cartridge-FIA systems show a reproducible operation, low pressure drop and long-range enzyme activities and are well suited for on-line monitoring of microbial cultivation processes.

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