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Involvement of arginine residues in the catalytic activity of catechol-O-methyltransferase

✍ Scribed by Tunnicliff, Godfrey; Ngo, That T.


Book ID
122070732
Publisher
Elsevier Science
Year
1979
Tongue
English
Weight
315 KB
Volume
10
Category
Article
ISSN
0306-3623

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Validation of assay of catechol-O-methyl
✍ PΓ€ivi Tuomainen; Ilkka ReenilΓ€; Pekka T. MΓ€nnistΓΆ πŸ“‚ Article πŸ“… 1996 πŸ› Elsevier Science 🌐 English βš– 570 KB

The multistep assay of specific catechol-O-methyltransferase (COMT) activity in human erythrocytes was validated. Enzyme preparations from lysed erythrocytes were incubated with a substrate (3,4-dihydroxybenzoic acid) in the presence of Mg2+ and S-adenosylmethionine. The reaction products (vanillic