No intracellular enzyme possessing milk-clotting activity was found in the mycelium of Aspergilha niger. There was a time-lag in the clotting stage on using small amounts of the culture filtrate in the reaction mixture. Dilution of milk caused a decrease in the milkclotting activity and this was att
Investigations on biochemical properties of milk-clotting enzymes
โ Scribed by Braun, I. ;Kunath, H.
- Publisher
- John Wiley and Sons
- Year
- 1988
- Tongue
- English
- Weight
- 308 KB
- Volume
- 32
- Category
- Article
- ISSN
- 0027-769X
No coin nor oath required. For personal study only.
โฆ Synopsis
The properties of proteolytic enzymes produces from calf maws and from an Ascomycete were studied. Both milk-clotting proteases have their optimum activity at pH 5.2 and 45 "C. The microbiological rennin has a second maximum activity at pH 3.5 and 55 "C. Temperatures above 55 "C cause a rapid decrease of activity.
The behaviour of enzyme activity is similar with varying substrate and enzyme concentrations. However, increasing amounts of enzyme in ratio to the substrate lead to reaction rates of the calf rennin differing clearly from that of the microbiological rennet complex.
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