The effect of D-glucose on milk clotting activity of Mucor miehei in a chemostat with biomass retention
✍ Scribed by Haluk Beyenal; Şule Şeker; Bekir Salih; Abdurrahman Tanyolaç
- Publisher
- Wiley (John Wiley & Sons)
- Year
- 1999
- Tongue
- English
- Weight
- 117 KB
- Volume
- 74
- Category
- Article
- ISSN
- 0268-2575
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✦ Synopsis
In this study microbial production of rennin from a commercial strain of Mucor miehei (NRRL 3420) has been accomplished in a continuously fed fermenter. The effects of feed D-glucose concentration (2.5±30 g dm À3 ) on milk clotting activity and on other system variables were investigated at optimum mixing and dilution rates of 400 rpm and 0.0052 h À1 , respectively, without pH control. Maximum milk clotting activity (1.24 IU cm À3 ) occurred when the feed D-glucose concentration was 7.5 g dm À3 . Enzyme production continued for 500 h, producing a total milk clotting activity of 10 230 IU. At the maximum milk clotting activity point, total protein concentration, milk clotting and proteolytic activities were analysed and compared with those of a commercial rennet. The ratio of milk clotting activity to proteolytic activity, and speci®c milk clotting activity were determined as 1.55 Â 10 À3 IU PU À1 and 5.28 IU mg À1 medium protein, respectively, denoting similar characteristics to a commercial rennet after concentration of the fermentation medium.