On the clotting of milk by rennet-like enzyme of Aspergillus niger

✍ Scribed by Dr. A. F. Abdel-Fattah; S. S. Mabrouk

• Publisher: John Wiley and Sons
• Year: 2007
• Tongue: English
• Weight: 388 KB
• Volume: 12
• Category: Article
• ISSN: 0233-111X
• DOI: 10.1002/jobm.19720120202

✦ Synopsis

No intracellular enzyme possessing milk-clotting activity was found in the mycelium of Aspergilha niger. There was a time-lag in the clotting stage on using small amounts of the culture filtrate in the reaction mixture. Dilution of milk caused a decrease in the milkclotting activity and this was attributed to several factors. Phosphate buffer was found t o accelerate the enzymatic coagulation of milk when the reaction mixture had a p H less than 6.3. The addition of CaCl, to buffered reaction mixtures caused decrease in pH and time of milk-clotting. The extent of any decrease depended on the amount of CaC1, added, the type and p H of buffer.