Investigation on clotting and hemolysis characteristics of heparin-immobilized polyether sulfones biomembrane
✍ Scribed by Changjun Hou; Quan Yuan; Danqun Huo; Shujia Zheng; Dongli Zhan
- Publisher
- John Wiley and Sons
- Year
- 2008
- Tongue
- English
- Weight
- 118 KB
- Volume
- 85A
- Category
- Article
- ISSN
- 1549-3296
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✦ Synopsis
Abstract
In this study, a novel heparin‐immobilized polyethersulfone (PES) was synthesized. PES was initially sulfonated with chlorosulfonic acid and then 1,6‐hexanediamine was grafted to the SO~3~H groups of sulfonated PES, which subsequently reacted with heparin through a covalent bond by using (1‐ethyl‐3‐(3‐dimethylaminopropyl) carbodiimide hydrochloride (EDAC) as catalyst. The hydrophobic/hydrophilic property was characterized by measuring the water contact angle. The data shows decline from 62.29° ± 1.2° to 47.86° ± 0.3° for water and 86.79° ± 0.8° to 68.34° ± 1.0° for glycerol, which indicates an enhancement of hydrophilicity. Plasma hemolysis assay shows a comparatively low hemolysis ratio of 1.04%, which is below permissible limit of 5%. A higher content of dissociated blood cells and Ca^2+^ concentration was found in red blood cell counting and coagulation factor IV test in heparinized PES. Plasma recalcification time of 360 s also offers positive evidence that heparinized PES seems to have a good anticoagulation property. This new heparin‐immobilized PES biomaterials may have the potential for biomedical applications. © 2007 Wiley Periodicals, Inc. J Biomed Mater Res, 2008