Investigation of the mechanisms of irreversible thermoinactivation of Bacillus stearothermophilusα-amylase
✍ Scribed by Marian P. BROSNAN; Catherine T. KELLY; William M. FOGARTY
- Book ID
- 115129803
- Publisher
- John Wiley and Sons
- Year
- 1992
- Tongue
- English
- Weight
- 754 KB
- Volume
- 203
- Category
- Article
- ISSN
- 1432-1327
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The synthesis and localization of a-amylase were studied in Bacillus stearothermophilus AN002 at 40,45,50 and 55 "C and also during early exponential, late exponential and stationary phases of growth. The extracellular a-amylase synthesis was highest at 50 "C and at stationary growth phase. Accumula
A 6.4 Kb HindIII fragment of Bacillus stearothermophilus DY-5 DNA cloned in Escherichia coli using pBR322 as a vector was shown to direct the synthesis of a thermophilic alpha-amylase. In attempts to reduce the size of the insert, the alpha-amylase gene was shown to be contained in a 3.1 Kb HindIII