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Cloning of a chromosomal α-amylase gene from Bacillus stearothermophilus

✍ Scribed by Per Linå Jørgensen; Gitte Bak Poulsen; Børge Diderichsen

Book ID
109316798
Publisher
John Wiley and Sons
Year
1991
Tongue
English
Weight
460 KB
Volume
77
Category
Article
ISSN
0378-1097

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Cloning and expression of a thermophilic
Cloning and expression of a thermophilic α-amylase gene from Bacillus stearothermophilus in Escherichia coli
✍ Tsukagoshi, N. ;Ihara, H. ;Yamagata, H. ;Udaka, S. 📂 Article 📅 1984 🏛 Springer 🌐 English ⚖ 584 KB

A 6.4 Kb HindIII fragment of Bacillus stearothermophilus DY-5 DNA cloned in Escherichia coli using pBR322 as a vector was shown to direct the synthesis of a thermophilic alpha-amylase. In attempts to reduce the size of the insert, the alpha-amylase gene was shown to be contained in a 3.1 Kb HindIII