Cloning and expression of a thermophilic
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Tsukagoshi, N. ;Ihara, H. ;Yamagata, H. ;Udaka, S.
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Article
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1984
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Springer
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English
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A 6.4 Kb HindIII fragment of Bacillus stearothermophilus DY-5 DNA cloned in Escherichia coli using pBR322 as a vector was shown to direct the synthesis of a thermophilic alpha-amylase. In attempts to reduce the size of the insert, the alpha-amylase gene was shown to be contained in a 3.1 Kb HindIII