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Cloning and characterization of a gene cluster from Bacillus stearothermophilus comprising infC, rpmI and rplT

โœ Scribed by Pon, Cynthia L. ;Brombach, Martin ;Thamm, Sabine ;Gualerzi, Claudio O.


Publisher
Springer
Year
1989
Tongue
English
Weight
268 KB
Volume
218
Category
Article
ISSN
0026-8925

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Cloning and expression of a thermophilic
โœ Tsukagoshi, N. ;Ihara, H. ;Yamagata, H. ;Udaka, S. ๐Ÿ“‚ Article ๐Ÿ“… 1984 ๐Ÿ› Springer ๐ŸŒ English โš– 584 KB

A 6.4 Kb HindIII fragment of Bacillus stearothermophilus DY-5 DNA cloned in Escherichia coli using pBR322 as a vector was shown to direct the synthesis of a thermophilic alpha-amylase. In attempts to reduce the size of the insert, the alpha-amylase gene was shown to be contained in a 3.1 Kb HindIII