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α-factor-directed synthesis of Bacillus stearothermophilus α-amylase in saccharomyces cerevisiae

✍ Scribed by Roberto V. Nonato; Kazuo Shishido


Book ID
117058163
Publisher
Elsevier Science
Year
1988
Tongue
English
Weight
604 KB
Volume
152
Category
Article
ISSN
0006-291X

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Expression kinetics of the human Epidermal Growth Factor (hEGF) from the a-factor prepro region in a 2-pm based plasmid was studied in Saccharomyces cerevisiae. Production o f hEGF was highly medium dependent as a chemically defined, nonenriched media had a significantly lower yield than did enriche