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Cloning and expression of the α-amylase gene from Bacillus stearothermophilus in several staphylococcal species

✍ Scribed by Thudt, Karin; Schleifer, Karl Heinz; Götz, Friedrich

Book ID
123066462
Publisher
Elsevier Science
Year
1985
Tongue
English
Weight
728 KB
Volume
37
Category
Article
ISSN
0378-1119

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Cloning and expression of a thermophilic
Cloning and expression of a thermophilic α-amylase gene from Bacillus stearothermophilus in Escherichia coli
✍ Tsukagoshi, N. ;Ihara, H. ;Yamagata, H. ;Udaka, S. 📂 Article 📅 1984 🏛 Springer 🌐 English ⚖ 584 KB

A 6.4 Kb HindIII fragment of Bacillus stearothermophilus DY-5 DNA cloned in Escherichia coli using pBR322 as a vector was shown to direct the synthesis of a thermophilic alpha-amylase. In attempts to reduce the size of the insert, the alpha-amylase gene was shown to be contained in a 3.1 Kb HindIII