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Cloning and expression of an amylase gene from Bacillus stearothermophilus

✍ Scribed by Diderichsen, B.; Poulsen, G.B.; LinåJørgensen, P.


Book ID
123296931
Publisher
Elsevier Science
Year
1991
Tongue
English
Weight
187 KB
Volume
142
Category
Article
ISSN
0923-2508

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Cloning and expression of a thermophilic
✍ Tsukagoshi, N. ;Ihara, H. ;Yamagata, H. ;Udaka, S. 📂 Article 📅 1984 🏛 Springer 🌐 English ⚖ 584 KB

A 6.4 Kb HindIII fragment of Bacillus stearothermophilus DY-5 DNA cloned in Escherichia coli using pBR322 as a vector was shown to direct the synthesis of a thermophilic alpha-amylase. In attempts to reduce the size of the insert, the alpha-amylase gene was shown to be contained in a 3.1 Kb HindIII